marker_id	species_id	marker_name	sub_number	marker_type	mapped_genome_id	reference_seq_name	chr	fwd_primer_seq	fwd_primer_start_pos	fwd_primer_end_pos	rev_primer_seq	rev_primer_start_pos	rev_primer_end_pos	target_seq	target_chr	target_start_pos	target_end_pos	allele	enzyme	comments	doi	pubmed	f_active	assembly_version
t38727.P0000001	t38727	ISSR-UBC812	1	Others	t38727.G001	P.virgatum_v5	NA	GAGAGAGAGAGAGAGAA	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000002	t38727	ISSR-UBC827	1	Others	t38727.G001	P.virgatum_v5	NA	ACACACACACACACACG	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000003	t38727	ISSR-UBC828	1	Others	t38727.G001	P.virgatum_v5	NA	TGTGTGTGTGTGTGTGA	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000004	t38727	ISSR-UBC829	1	Others	t38727.G001	P.virgatum_v5	NA	TGTGTGTGTGTGTGTGC	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000005	t38727	ISSR-UBC830	1	Others	t38727.G001	P.virgatum_v5	NA	TGTGTGTGTGTGTGTGG	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000006	t38727	ISSR-UBC835	1	Others	t38727.G001	P.virgatum_v5	NA	AGAGAGAGAGAGAGAGYC	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000007	t38727	ISSR-UBC836	1	Others	t38727.G001	P.virgatum_v5	NA	AGAGAGAGAGAGAGAGYT	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000008	t38727	ISSR-UBC844	1	Others	t38727.G001	P.virgatum_v5	NA	CTCTCTCTCTCTCTCTRC	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000009	t38727	ISSR-UBC848	1	Others	t38727.G001	P.virgatum_v5	NA	CACACACACACACACARG	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000010	t38727	ISSR-UBC854	1	Others	t38727.G001	P.virgatum_v5	NA	TCTCTCTCTCTCTCTCRG	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000011	t38727	ISSR-UBC868	1	Others	t38727.G001	P.virgatum_v5	NA	GAAGAAGAAGAAGAAGAA	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000012	t38727	ISSR-UBC876	1	Others	t38727.G001	P.virgatum_v5	NA	GATAGATAGACAGACA	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000013	t38727	ISSR-UBC879	1	Others	t38727.G001	P.virgatum_v5	NA	CTTCACTTCACTTCA	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000014	t38727	ISSR-UBC887	1	Others	t38727.G001	P.virgatum_v5	NA	DVDTCTCTCTCTCTCTC	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000015	t38727	ISSR-UBC890	1	Others	t38727.G001	P.virgatum_v5	NA	VHVGTGTGTGTGTGTGT	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000016	t38727	ISSR-UBC891	1	Others	t38727.G001	P.virgatum_v5	NA	HVHTGTGTGTGTGTGTG	0	0	NA	0	0	NA	NA	0	0	NA	NA	ISSR	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000017	t38727	SCoT2	1	Others	t38727.G001	P.virgatum_v5	NA	CAACAATGGCTACCACCC	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000018	t38727	SCoT3	1	Others	t38727.G001	P.virgatum_v5	NA	CAACAATGGCTACCACCG	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000019	t38727	SCoT4	1	Others	t38727.G001	P.virgatum_v5	NA	CAACAATGGCTACCACCT	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000020	t38727	SCoT5	1	Others	t38727.G001	P.virgatum_v5	NA	CAACAATGGCTACCACGA	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000021	t38727	SCoT6	1	Others	t38727.G001	P.virgatum_v5	NA	CAACAATGGCTACCACGC	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000022	t38727	SCoT7	1	Others	t38727.G001	P.virgatum_v5	NA	CAACAATGGCTACCACGG	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000023	t38727	SCoT9	1	Others	t38727.G001	P.virgatum_v5	NA	CAACAATGGCTACCAGCA	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000024	t38727	SCoT10	1	Others	t38727.G001	P.virgatum_v5	NA	CAACAATGGCTACCAGCC	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000025	t38727	SCoT12	1	Others	t38727.G001	P.virgatum_v5	NA	ACGACATGGCGACCAACG	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000026	t38727	SCoT13	1	Others	t38727.G001	P.virgatum_v5	NA	ACGACATGGCGACCATCG	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000027	t38727	SCoT15	1	Others	t38727.G001	P.virgatum_v5	NA	ACGACATGGCGACCGCGA	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000028	t38727	SCoT16	1	Others	t38727.G001	P.virgatum_v5	NA	ACCATGGCTACCACCGAC	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000029	t38727	SCoT18	1	Others	t38727.G001	P.virgatum_v5	NA	ACCATGGCTACCACCGCC	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000030	t38727	SCoT21	1	Others	t38727.G001	P.virgatum_v5	NA	ACGACATGGCGACCCACA	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000031	t38727	SCoT28	1	Others	t38727.G001	P.virgatum_v5	NA	CCATGGCTACCACCGCCA	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000032	t38727	SCoT31	1	Others	t38727.G001	P.virgatum_v5	NA	CCATGGCTACCACCGCCT	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000033	t38727	SCoT34	1	Others	t38727.G001	P.virgatum_v5	NA	ACCATGGCTACCACCGCA	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000034	t38727	SCoT35	1	Others	t38727.G001	P.virgatum_v5	NA	CATGGCTACCACCGGCCC	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000035	t38727	SCoT37	1	Others	t38727.G001	P.virgatum_v5	NA	CAATGGCTACCACTAGCC	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000036	t38727	SCoT48	1	Others	t38727.G001	P.virgatum_v5	NA	ACAATGGCTACCACTGGC	0	0	NA	0	0	NA	NA	0	0	NA	NA	SCoT	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000037	t38727	EST-SSR-cnl35	1	Others	t38727.G001	P.virgatum_v5	NA	AAGTGAGCACAACGACACGA	0	0	CGATCCAAAGAAGCAAAGATG	0	0	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000038	t38727	EST-SSR-cnl37	1	Others	t38727.G001	P.virgatum_v5	NA	CTGCCTCGCGTGAAAGATA	0	0	CCTCCTCGATCTGGATGGT	0	0	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000039	t38727	EST-SSR-cnl42	1	Others	t38727.G001	P.virgatum_v5	NA	GTTGGTCTGCTGCTCACTCG	0	0	CCGACGATGTTGAAGGAGAG	0	0	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000040	t38727	EST-SSR-cnl47	1	Others	t38727.G001	P.virgatum_v5	NA	GACTCGCACGATTTCTCCTCGCCAGACAACCAATTCAGGT	0	0	GCCAGACAACCAATTCAGGT	0	0	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000041	t38727	EST-SSR-cnl51	1	Others	t38727.G001	P.virgatum_v5	NA	CTAGGGTTTCCCACCTCTCA	0	0	AATGTCCTTGGCGTTGCT	0	0	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000042	t38727	EST-SSR-cnl55	1	Others	t38727.G001	P.virgatum_v5	NA	GCTGATAGCGAGGTGGGTAG	0	0	CTGCCGGTTGATCTTGTTCT	0	0	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000043	t38727	EST-SSR-cnl61	1	Others	t38727.G001	P.virgatum_v5	NA	CACGAGTGCAGAGCTAGACG	0	0	ACAACAACCCGACTGCTACC	0	0	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000044	t38727	EST-SSR-cnl86	1	Others	t38727.G001	P.virgatum_v5	NA	CAACAACGTCAACGCCTTC	0	0	GCGTCTTGAACCTCTTGTCC	0	0	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000045	t38727	EST-SSR-cnl100	1	Others	t38727.G001	P.virgatum_v5	NA	CGTCGTCCTCTGCTGTGAG	0	0	AGGTCGTCCATCTGCTGCT	0	0	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000046	t38727	EST-SSR-cnl115	1	Others	t38727.G001	P.virgatum_v5	NC_053142.1	CGAGAAGAAGGTGGTGTCGT	35672994	35673013	AGGTCGTGGAAGGTCTTGG	35673200	35673182	NA	NA	0	0	NA	NA	Blast_hit_num.:2	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000047	t38727	EST-SSR-cnl119	1	Others	t38727.G001	P.virgatum_v5	NA	ATCGTCTCCTCCTCCTCCA	0	0	ATGCCTCGGTGGACTGGTA	0	0	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000048	t38727	EST-SSR-cnl130	1	Others	t38727.G001	P.virgatum_v5	NA	AAATGTTGAGCAACGGGAGCT	0	0	ACTTCATAGGGCGGAGGTCT	0	0	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000049	t38727	EST-SSR-cnl144	1	Others	t38727.G001	P.virgatum_v5	NC_053147.1	AGAAGGCGGCTCAGAAGAAG	52534659	52534678	GCTCCAACTCAGAATCAACAA	52535905	52535885	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000050	t38727	EST-SSR-cnl147	1	Others	t38727.G001	P.virgatum_v5	NA	GGCTAGGGTTTCGACTCCTC	0	0	AGATGGCGAACTCGACCTG	0	0	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000051	t38727	EST-SSR-cnl158	1	Others	t38727.G001	P.virgatum_v5	NA	CTCATCCCACCACCACCAC	0	0	CCCTGAAGAAGTCGAACACG	0	0	NA	NA	0	0	NA	NA	NA	10.1186/s41065-016-0007-z	28096766	1	P.virgatum_v5
t38727.P0000384	t38727	4KG163000	1	Others	t38727.G001	P.virgatum_v5	NA	gcagagctcATGAATTATA	0	0	tgcggatccTCAGAACCAT	0	0	NA	NA	0	0	NA	SacI, BamHI	NA	10.1093/jxb/erad255	37402629	1	P.virgatum_v5
t38727.P0000385	t38727	pS-OX	1	Others	t38727.G001	P.virgatum_v5	NA	CAGTGGTCCCAAAGATGGAC	0	0	CCGCCAATATATCCTGTCA	0	0	NA	NA	0	0	NA	NA	NA	10.1093/jxb/erad255	37402629	1	P.virgatum_v5
t38727.P0000386	t38727	PvHd1	1	Others	t38727.G001	P.virgatum_v5	NC_053139.1	TCAGTGCTTACACCGATTCC	19029349	19029366	TCAGAACCATGGGACAGT	19030534	19030515	NA	NA	0	0	NA	NA	Blast_hit_num.:2	10.1093/jxb/erad255	37402629	1	P.virgatum_v5
t38727.P0000387	t38727	ACN	1	Others	t38727.G001	P.virgatum_v5	NA	ACCTTTAACTCTCCCGCTA	0	0	CAAGGTCAAGACGGAGGAT	0	0	NA	NA	0	0	NA	NA	NA	10.1093/jxb/erad255	37402629	1	P.virgatum_v5
t38727.P0000388	t38727	PvHd1_CDS_F / PvHd1_qPCR_R	1	Others	t38727.G001	P.virgatum_v5	NC_053139.1	ATGAATTATAATTTTGGCAG	19031158	19031176	CAGAGGTACGCAGCGTCAG	19031297	19031278	NA	NA	0	0	NA	NA	NA	10.1093/jxb/erad255	37402629	1	P.virgatum_v5
t38727.P0000389	t38727	attB1-pvHd1_F / attB2-pvHd1_R	1	Others	t38727.G001	P.virgatum_v5	NA	GGGGACAAGTTTGTACAAAAAGCAGGCTCCATGAATTATAATTTTGGCAG	0	0	GGGGACCACTTTGTACAAGAAAGCTGGGTCTCAGAACCATGGGACAGT	0	0	NA	NA	0	0	NA	NA	NA	10.1093/jxb/erad255	37402629	1	P.virgatum_v5
t38727.P0000390	t38727	PvHd1_stdiff_R3	1	Others	t38727.G001	P.virgatum_v5	NA	NA	0	0	CAGAGTGCACCTTGGCGTCA	0	0	NA	NA	0	0	NA	NA	A 2 microlitter aliquot of the ligated products was used for transformation in E. coli 10beta, and single transformed colonies were tested by PCR for the presence of the insert using the primer set attB1_PvHd1_F/PvHd1_stdiff_R3 and Q5 High-Fidelity DNA polymerase (NEB).	10.1093/jxb/erad255	37402629	1	P.virgatum_v5